This practical and highly-interactive course includes practical sessions and exercises where participants will visit the laboratory and they will be introduced to various lab instruments and gas chromatography process. Practical sessions will be performed using one of the lab equipment in order to apply the theory learnt in the class.The purpose of this course is to provide training and insight in advanced applications and techniques of Gas Chromatography, along with their application to practical industry and laboratory problems. The course will include classroom instruction along with a question and answer session for problems encountered by the participants in the class as well as a one day hands on workshop to actively demonstrate the concepts covered in the theoretical seminar. All participants are encouraged to bring issues and concerns to the seminarfor discussion. Gas chromatography-Mass spectrometry (GC/MS) combines the fine separating power of GC with the uniquely powerful detection capabilities of MS. The GC/MS has been widely heralded as the “gold standard” in scientific analysis as Gas Chromatography/Mass Spectrometry combines the strengths of the two powerful analytical techniques. The GC/MS instrument is made up of two parts. The gas chromatography (GC) portion separates the chemical mixture into pulses of pure chemicals and the mass spectrometer (MS) identifies and quantifies the chemicals. This powerful technique is particularly suitable for the analysis of mixtures of volatile and low relative molecular mass compounds (<800) such as hydrocarbons, fragrances, essential oils and relatively non-polar drugs. Chemical derivatisation, e.g. trimethylsilylation, can often be employed to increase the volatility of compounds containing polar functional groups (-OH, -COOH, -NH2, etc.) thereby extending the range of suitable analytes to such compounds as steroids, polar drugs, prostaglandins, bile acids, organic acids, amino acids and small peptides. The course will introduce participants to the advanced principles and practices of gas chromatography and determine how the individual elements interrelate. Participants will be able to competently use and develop methods on a gas chromatograph to produce robust and valid analytical data. The course combines lecture, laboratory, and problem-solving sessions that use actual GC/MS experimental data acquired by the participants. Through the analysis of these data, you will learn important fundamental operational techniques and will solve common problems that require you to hone your interpretational and experimental skills.
Upon the successful completion of this course, each participant will be able to:-
DAY 1
Chromatography Theory & Applications
Capillarity Flow Technology
Multidimensional GC
Hyphenated Techniques with Spectral (2D) Detectors
DAY 2
GC/MS System
GC/MS Sample Preparation
GC/MS Systems
GC/MS Hardware
DAY 3
Mass Spectrometry
GC/MS Analysis Evaluation
MS Features of Substance Classes
Calibration & Quantitation
DAY 4
Travel to Laboratory
DAY 5
Validation of GC Methods
System Maintenance and Troubleshooting
This course provides an overview of all significant aspects and considerations of advanced of gas chromatography/mass spectrometry (GC/MS) for analytical chemists, chromatographers, mass spectrometrists, operators, researchers, biochemists and biotechnologists, specifically for those who work with gas chromatography and find that their future responsibilities combine gas chromatographic techniques with mass spectrometry and those who wish to expand their technical expertise by learning gas chromatography/mass spectrometry.